Hotspot

matK-trnK

LSC · 3451-4496 · 1046 bp

Back to Cattleya

Pi 0.0039

MarkerSeek score 62.80

Primer available yes

Label matK-trnK

Feature Metadata

label_name: matK-trnK
feature_type: igs
region: LSC
length_bp: 1046
variable_sites: 4
pi: 0.0039
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK_p1	AGGAAGTTTTGTTGCCGAAA	ATCCGGGTTGCTAACTCAAC	40.0	50.0	56.4	57.9	1158-1159 bp	1.0	67.8
matK-trnK_p2	AGGAAGTTTTGTTGCCGAAA	AACGGTAGAGTACTCGGCTT	40.0	50.0	56.4	58.2	1141-1142 bp	1.0	67.3
matK-trnK_p3	AGGAAGTTTTGTTGCCGAAA	ACGGTAGAGTACTCGGCTTT	40.0	50.0	56.4	58.2	1140-1141 bp	1.0	67.3
matK-trnK_p4	AGGAAGTTTTGTTGCCGAAA	GATCCGGGTTGCTAACTCAA	40.0	50.0	56.4	57.6	1159-1160 bp	1.0	65.5
matK-trnK_p5	AGGAAGTTTTGTTGCCGAAA	TTGATCCGGGTTGCTAACTC	40.0	50.0	56.4	57.6	1161-1162 bp	1.0	65.5