primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK_p1	matK-trnK	1	AGGAAGTTTTGTTGCCGAAA	ATCCGGGTTGCTAACTCAAC	20	20	40.0	50.0	56.4	57.9	0.0	0.0	1.737827	3451	4496	1158-1159	1.000000	67.8
matK-trnK_p2	matK-trnK	2	AGGAAGTTTTGTTGCCGAAA	AACGGTAGAGTACTCGGCTT	20	20	40.0	50.0	56.4	58.2	0.0	17.1	1.796822	3451	4496	1141-1142	1.000000	67.3
matK-trnK_p3	matK-trnK	3	AGGAAGTTTTGTTGCCGAAA	ACGGTAGAGTACTCGGCTTT	20	20	40.0	50.0	56.4	58.2	0.0	17.1	1.796822	3451	4496	1140-1141	1.000000	67.3
matK-trnK_p4	matK-trnK	4	AGGAAGTTTTGTTGCCGAAA	GATCCGGGTTGCTAACTCAA	20	20	40.0	50.0	56.4	57.6	0.0	0.0	2.024992	3451	4496	1159-1160	1.000000	65.5
matK-trnK_p5	matK-trnK	5	AGGAAGTTTTGTTGCCGAAA	TTGATCCGGGTTGCTAACTC	20	20	40.0	50.0	56.4	57.6	0.0	0.0	2.024992	3451	4496	1161-1162	1.000000	65.5
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TTCCTTCTCTTCGGGATCGA	GACCAAATCAAAATGGGGCG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.148075	5327	7201	2018-2070	1.000000	81.0
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TTCCTTCTCTTCGGGATCGA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.215424	5327	7201	1957-2009	1.000000	80.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GATGCCCCCGAAACGTATAG	GACCAAATCAAAATGGGGCG	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.216746	5327	7201	1945-1997	1.000000	80.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GATGCCCCCGAAACGTATAG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.2	58.1	0.0	16.9	0.284095	5327	7201	1884-1936	1.000000	80.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TTCCTTCTCTTCGGGATCGA	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	57.9	57.8	0.0	16.9	0.350676	5327	7201	1961-2013	1.000000	79.4
ndhK_p1	ndhK	1	TCTTTACGTAAAGGCCAGCC	GGGGATGCTTGGGTACAATT	20	20	50.0	50.0	57.9	57.8	35.6	0.0	0.262716	49570	50297	845-845	1.000000	78.2
ndhK_p2	ndhK	2	CGACACGACTCCAGATTTCA	GGGGATGCTTGGGTACAATT	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.506876	49570	50297	920-920	1.000000	76.2
ndhK_p3	ndhK	3	ACGACACGACTCCAGATTTC	GGGGATGCTTGGGTACAATT	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.508476	49570	50297	921-921	1.000000	76.2
ndhK_p4	ndhK	4	TCTTTACGTAAAGGCCAGCC	TGCTTGGGTACAATTCCGAA	20	20	50.0	45.0	57.9	57.4	35.6	0.0	0.673952	49570	50297	840-840	1.000000	74.9
ndhK_p5	ndhK	5	TCTTTACGTAAAGGCCAGCC	GGGTACAATTCCGAATTCGC	20	20	50.0	50.0	57.9	57.2	35.6	22.4	0.853838	49570	50297	835-835	1.000000	73.5
ndhC_p1	ndhC	1	TGAATTGGGGGTTGTTTGGT	TTCAGCCTACTCCACATTCA	20	20	45.0	45.0	57.8	56.2	0.0	0.0	2.023104	50180	50539	500-500	1.000000	63.8
ndhC_p2	ndhC	2	TGAATTGGGGGTTGTTTGGT	CAGCCTACTCCACATTCATAGG	20	22	45.0	50.0	57.8	58.0	0.0	0.0	2.182847	50180	50539	498-498	1.000000	62.5
ndhC_p3	ndhC	3	TGAATTGGGGGTTGTTTGGT	AGCCTACTCCACATTCATAGGA	20	22	45.0	45.5	57.8	58.1	0.0	0.0	2.253761	50180	50539	497-497	1.000000	62.0
ndhC_p4	ndhC	4	TGAATTGGGGGTTGTTTGGT	TCAGCCTACTCCACATTCAT	20	20	45.0	45.0	57.8	55.9	0.0	0.0	2.259164	50180	50539	499-499	1.000000	61.9
ndhC_p5	ndhC	5	TGAATTGGGGGTTGTTTGGT	TTCAGCCTACTCCACATTCAT	20	21	45.0	42.9	57.8	56.7	0.0	0.0	2.472190	50180	50539	500-500	1.000000	60.2
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CGGGGCTAAAATCCCAGAAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	0.1	0.0	0.335093	50540	51177	770-776	1.000000	77.7
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCTGGACCTTCCCTAATCGG	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.2	57.8	0.0	0.0	0.407760	50540	51177	787-793	1.000000	77.1
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	AATCGGGGCTAAAATCCCAG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.6	57.8	0.1	0.0	0.562255	50540	51177	773-779	1.000000	75.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CGGGGCTAAAATCCCAGAAA	AGCCCAATGCAACAAAATGC	20	20	50.0	45.0	57.9	58.5	0.1	0.0	0.611869	50540	51177	852-858	1.000000	75.5
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CGGGGCTAAAATCCCAGAAA	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.9	57.5	0.1	0.0	0.678865	50540	51177	769-775	1.000000	75.0
atpB-rbcL_p1	atpB-rbcL	1	TGCCCGGTGGAAAAATTACA	GGTGACATAAGTCCCTCCCT	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.122261	54182	55139	1085-1102	1.000000	80.8
atpB-rbcL_p2	atpB-rbcL	2	ATCTTGCCCGGTGGAAAAAT	GGTGACATAAGTCCCTCCCT	20	20	45.0	55.0	58.1	58.1	0.0	0.0	0.181868	54182	55139	1089-1106	1.000000	80.3
atpB-rbcL_p3	atpB-rbcL	3	TGAGTGATACGCCCTAGGTT	GGTGACATAAGTCCCTCCCT	20	20	50.0	55.0	57.8	58.1	8.3	0.0	0.273626	54182	55139	1044-1061	1.000000	79.6
atpB-rbcL_p4	atpB-rbcL	4	TTGAGTGATACGCCCTAGGT	GGTGACATAAGTCCCTCCCT	20	20	50.0	55.0	57.8	58.1	8.3	0.0	0.273626	54182	55139	1045-1062	1.000000	79.6
atpB-rbcL_p5	atpB-rbcL	5	TCTTGCCCGGTGGAAAAATT	GGTGACATAAGTCCCTCCCT	20	20	45.0	55.0	58.3	58.1	0.0	0.0	0.408243	54182	55139	1088-1105	1.000000	78.5
rbcL-accD_p1	rbcL-accD	1	CTAGCAAATGGAGCCCTGAG	CCACTGGTATGTCCAAGAGC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.065286	56604	57461	1042-1066	1.000000	81.4
rbcL-accD_p2	rbcL-accD	2	TGAAGCTAGCAAATGGAGCC	CCACTGGTATGTCCAAGAGC	20	20	50.0	55.0	58.2	58.0	18.8	0.0	0.274659	56604	57461	1047-1071	1.000000	79.7
rbcL-accD_p3	rbcL-accD	3	CGCTGCTTGTGAAGTATGGA	CCACTGGTATGTCCAAGAGC	20	20	50.0	55.0	58.3	58.0	0.0	0.0	0.304198	56604	57461	1017-1041	1.000000	79.5
rbcL-accD_p4	rbcL-accD	4	TTTCGACCCAGTGGATAAGC	CCACTGGTATGTCCAAGAGC	20	20	50.0	55.0	57.6	58.0	12.0	0.0	0.426428	56604	57461	981-1005	1.000000	78.6
rbcL-accD_p5	rbcL-accD	5	TTCGACCCAGTGGATAAGCT	CCACTGGTATGTCCAAGAGC	20	20	50.0	55.0	58.4	58.0	12.0	0.0	0.461334	56604	57461	980-1004	1.000000	78.3
clpP_p1	clpP	1	GCGGGTTGGTTTAGATCGAT	AAACCATTGTTACGTTTCCA	20	20	50.0	35.0	58.1	53.6	0.0	0.0	4.431422	70718	72960	2410-2412	1.000000	44.6
clpP_p2	clpP	2	GCGGGTTGGTTTAGATCGAT	AAAACCATTGTTACGTTTCCA	20	21	50.0	33.3	58.1	54.5	0.0	0.0	4.549010	70718	72960	2411-2413	1.000000	43.7
clpP_p3	clpP	3	GCGGGTTGGTTTAGATCGAT	AACCATTGTTACGTTTCCAT	20	20	50.0	35.0	58.1	53.4	0.0	0.0	4.674179	70718	72960	2409-2411	1.000000	42.7
clpP_p4	clpP	4	GCGGGTTGGTTTAGATCGAT	AAACCATTGTTACGTTTCCAT	20	21	50.0	33.3	58.1	54.3	0.0	0.0	4.777001	70718	72960	2410-2412	1.000000	41.9
clpP_p5	clpP	5	GCGGGTTGGTTTAGATCGAT	AAAACCATTGTTACGTTTCCAT	20	22	50.0	31.8	58.1	55.1	0.0	0.0	4.960855	70718	72960	2411-2413	1.000000	40.4
clpP-psbB_p1	clpP-psbB	1	CGCACTATACGTCAACCCAA	GGCCCAACCAGAAACTAGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.116294	72961	73920	1138-1142	1.000000	81.2
clpP-psbB_p2	clpP-psbB	2	TATACGTCAACCCAAGCTGC	GGCCCAACCAGAAACTAGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.133885	72961	73920	1133-1137	1.000000	81.0
clpP-psbB_p3	clpP-psbB	3	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.238244	72961	73920	1056-1060	1.000000	80.4
clpP-psbB_p4	clpP-psbB	4	TATACGTCAACCCAAGCTGC	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.255834	72961	73920	1051-1055	1.000000	80.2
clpP-psbB_p5	clpP-psbB	5	CTTTTGGAACACCAATGGGC	GGCCCAACCAGAAACTAGAG	20	20	50.0	55.0	57.8	57.9	3.6	0.0	0.283669	72961	73920	1084-1088	1.000000	79.9
petB-petD_p1	petB-petD	1	ATTGCCTCTTCTTACTGCCG	GATCCATCCCCCAAAGGAAC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.103090	77725	78716	1097-1098	1.000000	79.5
petB-petD_p2	petB-petD	2	AAGCAAGGTATTTCGGGTCC	GATCCATCCCCCAAAGGAAC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.197529	77725	78716	1045-1046	1.000000	78.8
petB-petD_p3	petB-petD	3	GCAAGGTATTTCGGGTCCTT	GATCCATCCCCCAAAGGAAC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.197529	77725	78716	1043-1044	1.000000	78.8
petB-petD_p4	petB-petD	4	ATTGCCTCTTCTTACTGCCG	ATGTCCCATCCCTTTAGCCA	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.422614	77725	78716	1184-1185	1.000000	77.0
petB-petD_p5	petB-petD	5	GCAAGGTATTTCGGGTCCTT	ATGTCCCATCCCTTTAGCCA	20	20	50.0	50.0	57.9	58.4	0.0	0.0	0.517053	77725	78716	1130-1131	1.000000	76.2
trnN-GUU-rpl32_p1	trnN-GUU-rpl32	1	GGCAGAATAGCAGAGCAAGT	GATTGTCCGGTGGAAATCGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054072	110129	111084	1491-1570	1.000000	81.8
trnN-GUU-rpl32_p2	trnN-GUU-rpl32	2	AAAGCGAGCCCTTTCATTCA	GATTGTCCGGTGGAAATCGA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.108842	110129	111084	1209-1288	1.000000	81.8
trnN-GUU-rpl32_p3	trnN-GUU-rpl32	3	AAGCGAGCCCTTTCATTCAA	GATTGTCCGGTGGAAATCGA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.108842	110129	111084	1208-1287	1.000000	81.8
trnN-GUU-rpl32_p4	trnN-GUU-rpl32	4	TCAAAGCGAGCCCTTTCATT	GATTGTCCGGTGGAAATCGA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.108842	110129	111084	1211-1290	1.000000	81.8
trnN-GUU-rpl32_p5	trnN-GUU-rpl32	5	GTAACCTGGGCTGGAATCAG	GATTGTCCGGTGGAAATCGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.062665	110129	111084	1556-1635	1.000000	81.6
ccsA-ndhD_p1	ccsA-ndhD	1	ATGCAGCTCTTTTATGCGGA	TTCTCGATGGCTTCTCTTGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.058422	113124	113399	1162-1163	1.000000	80.6
ccsA-ndhD_p2	ccsA-ndhD	2	TGCAGCTCTTTTATGCGGAT	TTCTCGATGGCTTCTCTTGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.058422	113124	113399	1161-1162	1.000000	80.6
ccsA-ndhD_p3	ccsA-ndhD	3	ATGCAGCTCTTTTATGCGGA	CTCGATGGCTTCTCTTGCAT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.091858	113124	113399	1160-1161	1.000000	80.3
ccsA-ndhD_p4	ccsA-ndhD	4	TGCAGCTCTTTTATGCGGAT	CTCGATGGCTTCTCTTGCAT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.091858	113124	113399	1159-1160	1.000000	80.3
ccsA-ndhD_p5	ccsA-ndhD	5	TGCAGCTCTTTTATGCGGAT	GGTTCTATAACTGGCACGGG	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.093221	113124	113399	1343-1344	1.000000	80.2
ndhD_p1	ndhD	1	TGTACAACGAAGAATAGTGAGA	CGACAAAAGCCTGTACTCGA	22	20	36.4	50.0	54.6	57.9	0.0	0.0	5.410707	113400	114904	1692-1695	1.000000	40.4
ndhD_p2	ndhD	2	TGTACAACGAAGAATAGTGAGA	ACCGACAAAAGCCTGTACTC	22	20	36.4	50.0	54.6	57.8	0.0	0.0	5.515700	113400	114904	1694-1697	1.000000	40.4
ndhD_p3	ndhD	3	TGTACAACGAAGAATAGTGAGA	CACGGGCTTTTCTGGTCTAA	22	20	36.4	50.0	54.6	57.8	0.0	0.0	5.529293	113400	114904	1642-1645	1.000000	40.4
ndhD_p4	ndhD	4	TGTACAACGAAGAATAGTGAGAA	CGACAAAAGCCTGTACTCGA	23	20	34.8	50.0	55.4	57.9	0.0	0.0	5.648809	113400	114904	1692-1695	1.000000	40.4
ndhD_p5	ndhD	5	TTGTACAACGAAGAATAGTGAGA	CGACAAAAGCCTGTACTCGA	23	20	34.8	50.0	55.4	57.9	3.6	0.0	5.648809	113400	114904	1693-1696	1.000000	40.4
ndhE_p1	ndhE	1	GGACAAGCGGATTCACATCT	ATGGTGTTCTTCGGCTGATC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.059677	115768	116073	1330-1397	1.000000	83.8
ndhE_p2	ndhE	2	AATACATCCGTGGGACAAGC	ATGGTGTTCTTCGGCTGATC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.068225	115768	116073	1231-1298	1.000000	83.8
ndhE_p3	ndhE	3	AATACATCCGTGGGACAAGC	ACCCATCAATGCATGGTGTT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.097010	115768	116073	1243-1310	1.000000	83.6
ndhE_p4	ndhE	4	GGACAAGCGGATTCACATCT	ACCCATCAATGCATGGTGTT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.088461	115768	116073	1342-1409	1.000000	83.5
ndhE_p5	ndhE	5	GGACAAGCGGATTCACATCT	TGACTGTACCGGCTACAAGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.116060	115768	116073	1396-1463	1.000000	83.2
ndhE-ndhG_p1	ndhE-ndhG	1	CCATGCTTTGGCTTGTGATG	AGGTGCAATTACTATGGCTCG	20	21	50.0	47.6	58.0	58.2	0.0	0.0	1.165847	116074	116269	291-303	1.000000	75.0
ndhE-ndhG_p2	ndhE-ndhG	2	TGCTTTGGCTTGTGATGAGT	AGGTGCAATTACTATGGCTCG	20	21	45.0	47.6	58.0	58.2	0.0	0.0	1.175791	116074	116269	288-300	1.000000	74.9
ndhE-ndhG_p3	ndhE-ndhG	3	CCATGCTTTGGCTTGTGATG	GCCTTATGTAACACGTTGGG	20	20	50.0	50.0	58.0	56.8	0.0	0.0	1.200957	116074	116269	379-391	1.000000	73.7
ndhE-ndhG_p4	ndhE-ndhG	4	TGCTTTGGCTTGTGATGAGT	GCCTTATGTAACACGTTGGG	20	20	45.0	50.0	58.0	56.8	0.0	0.0	1.210901	116074	116269	376-388	1.000000	73.7
ndhE-ndhG_p5	ndhE-ndhG	5	CCATGCTTTGGCTTGTGATG	GCCTTATGTAACACGTTGGGA	20	21	50.0	47.6	58.0	58.3	0.0	0.0	1.302322	116074	116269	379-391	1.000000	72.9
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	TAGTGAAAATAGGCGACGGC	GGCAGAATAGCAGAGCAAGT	20	20	50.0	50.0	58.1	58.0	14.4	0.0	0.104963	123626	123965	1105-1459	1.000000	85.3
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	TAGTGAAAATAGGCGACGGC	GTAACCTGGGCTGGAATCAG	20	20	50.0	55.0	58.1	58.0	14.4	0.0	0.113555	123626	123965	1170-1524	1.000000	85.1
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	CCTTCTTCCATAACCCGAGC	GGCAGAATAGCAGAGCAAGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.080603	123626	123965	905-905	0.500000	59.4
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	CCTTCTTCCATAACCCGAGC	GTAACCTGGGCTGGAATCAG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.089196	123626	123965	970-970	0.500000	59.3
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	CCTTCTTCCATAACCCGAGC	AAGCGAGCCCTTTCATTCAA	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.135373	123626	123965	622-622	0.500000	58.9
rps19_p1	rps19	1	AAAAGGAGGAGTAATCAGCC	AGGCTTTTCACATGAATACT	20	20	45.0	35.0	54.7	52.4	0.0	0.0	8.849660	147948	148226	330-333	1.000000	42.9
rps19_p2	rps19	2	AAAAGGAGGAGTAATCAGCC	AAGGCTTTTCACATGAATACT	20	21	45.0	33.3	54.7	53.4	0.0	0.0	8.898980	147948	148226	331-334	1.000000	42.9
rps19_p3	rps19	3	AAAAGGAGGAGTAATCAGCC	AGGCTTTTCACATGAATACTT	20	21	45.0	33.3	54.7	53.4	0.0	0.0	8.898980	147948	148226	330-333	1.000000	42.9
rps19_p4	rps19	4	AAAAGGAGGAGTAATCAGCC	AGGCTTTTCACATGAATACTTAGT	20	24	45.0	33.3	54.7	56.4	0.0	0.0	8.927864	147948	148226	330-333	1.000000	42.9
rps19_p5	rps19	5	AAAAAGGAGGAGTAATCAGCC	AGGCTTTTCACATGAATACT	21	20	42.9	35.0	55.6	52.4	0.0	0.0	9.011084	147948	148226	331-334	1.000000	42.9