Hotspot

ndhD

SSC · 2292-3821 · 1530 bp

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Pi 0.00261

MarkerSeek score 47.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 6
pi: 0.00261
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AAGACTTTCCTTCCGTCTCA	TATTTTGAGGACGGGCTTTT	45.0	40.0	56.1	55.2	1581 bp	1.0	43.1
ndhD_p2	AAGACTTTCCTTCCGTCTCA	TTATTTTGAGGACGGGCTTT	45.0	40.0	56.1	55.2	1582 bp	1.0	43.1
ndhD_p3	AAGACTTTCCTTCCGTCTCA	TTATTTTGAGGACGGGCTTTT	45.0	38.1	56.1	56.0	1582 bp	1.0	41.6
ndhD_p4	AGACTTTCCTTCCGTCTCAT	TATTTTGAGGACGGGCTTTT	45.0	40.0	55.9	55.2	1580 bp	1.0	41.2
ndhD_p5	AGACTTTCCTTCCGTCTCAT	TTATTTTGAGGACGGGCTTT	45.0	40.0	55.9	55.2	1581 bp	1.0	41.2