Hotspot

atpF

LSC · 53950-55345 · 1396 bp

Back to Catopsis

Pi 0.00335

MarkerSeek score 50.70

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1396
variable_sites: 7
pi: 0.00335
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCGGCTCGAA	ACTTTGTTCTTTGTCAGTCCT	45.0	38.1	57.4	55.3	1539-1563 bp	1.0	45.8
atpF_p2	ACTAATTTCGTCGGCTCGAA	AGAACTTTGTTCTTTGTCAGTCC	45.0	39.1	57.4	57.2	1542-1566 bp	1.0	45.0
atpF_p3	ACTAATTTCGTCGGCTCGAA	GAACTTTGTTCTTTGTCAGTCCT	45.0	39.1	57.4	57.2	1541-1565 bp	1.0	45.0
atpF_p4	ACTAATTTCGTCGGCTCGAA	AACTTTGTTCTTTGTCAGTCCT	45.0	36.4	57.4	56.1	1540-1564 bp	1.0	44.0
atpF_p5	GGCTCGAAGGGTTACCATTA	ACTTTGTTCTTTGTCAGTCCT	50.0	38.1	57.0	55.3	1527-1551 bp	1.0	42.9