Hotspot

ycf1-ndhF

SSC · 155349-155357 · 9 bp

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Pi 0.296

MarkerSeek score 72.70

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 9
variable_sites: 4
pi: 0.296
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACAGGAACAAGAGGGATCCA	ACCGTGGGGATAAGAGGATT	50.0	50.0	58.0	57.8	503 bp	0.666667	65.1
ycf1-ndhF_p2	AAGAGGGATCCACCGAAGAA	ACCGTGGGGATAAGAGGATT	50.0	50.0	58.0	57.8	495 bp	0.666667	65.1
ycf1-ndhF_p3	ACAGGAACAAGAGGGATCCA	GAGGTGGACGAATCTCCTCT	50.0	55.0	58.0	58.2	382 bp	0.666667	64.9
ycf1-ndhF_p4	AAGAGGGATCCACCGAAGAA	GAGGTGGACGAATCTCCTCT	50.0	55.0	58.0	58.2	374 bp	0.666667	64.9
ycf1-ndhF_p5	ACAGGAACAAGAGGGATCCA	AGGTGGACGAATCTCCTCTT	50.0	50.0	58.0	57.8	381 bp	0.666667	64.8