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rps11-rpl36

LSC · 124040-124180 · 141 bp

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Pi 0.0189

MarkerSeek score 62.70

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 141
variable_sites: 4
pi: 0.0189
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	GAACCAATACGCCCATTCCT	GCAGGCGTGGACGAATTATA	50.0	50.0	57.9	58.1	256 bp	1.0	79.7
rps11-rpl36_p2	AATACGCCCATTCCTACGTG	GCAGGCGTGGACGAATTATA	50.0	50.0	57.8	58.1	251 bp	1.0	78.3
rps11-rpl36_p3	CAATACGCCCATTCCTACGT	GCAGGCGTGGACGAATTATA	50.0	50.0	57.8	58.1	252 bp	1.0	78.3
rps11-rpl36_p4	TGAACCAATACGCCCATTCC	GCAGGCGTGGACGAATTATA	50.0	50.0	58.2	58.1	257 bp	1.0	78.2
rps11-rpl36_p5	TTACGTGAACCAATACGCCC	GCAGGCGTGGACGAATTATA	50.0	50.0	58.3	58.1	262 bp	1.0	77.9