Hotspot

trnT-GGU-psbD

LSC · 75301-76350 · 1050 bp

Back to Catopsis

Pi 0.00889
MarkerSeek score 55.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1050
variable_sites
14
pi
0.00889
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 55.0 50.0 58.1 58.1 1208-1212 bp 1.0 79.6
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1211-1215 bp 1.0 79.5
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1198-1202 bp 1.0 78.6
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1201-1205 bp 1.0 78.4
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG CCCTTCGTAACCAGTCATCC 55.0 55.0 58.1 57.7 1182-1186 bp 1.0 78.1