Hotspot

trnC-GCA-petN

LSC · 70686-71726 · 1041 bp

Back to Catopsis

Pi 0.0157
MarkerSeek score 69.40
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
1041
variable_sites
24
pi
0.0157
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 AGTTCAAATCTGGGTGTCGC AGTCCACTCCTTCCCCATAC 50.0 55.0 58.5 58.1 1149-1152 bp 1.0 77.3
trnC-GCA-petN_p2 AGTTCAAATCTGGGTGTCGC GTCCACTCCTTCCCCATACT 50.0 55.0 58.5 58.1 1148-1151 bp 1.0 77.3
trnC-GCA-petN_p3 AGTTCAAATCTGGGTGTCGC AGCCCAAGCGAGACTTACTA 50.0 50.0 58.5 58.2 1090-1093 bp 1.0 77.2
trnC-GCA-petN_p4 AGTTCAAATCTGGGTGTCGC GAGTCCACTCCTTCCCCATA 50.0 55.0 58.5 57.8 1150-1153 bp 1.0 76.9
trnC-GCA-petN_p5 AGTTCAAATCTGGGTGTCGC CTAGAGTCCACTCCTTCCCC 50.0 60.0 58.5 58.2 1153-1156 bp 1.0 76.5