Hotspot

rps19-psbA

LSC · 41942-42090 · 149 bp

Back to Catopsis

Pi 0.027

MarkerSeek score 69.70

Primer available yes

Label rps19-psbA

Feature Metadata

label_name: rps19-psbA
feature_type: igs
region: LSC
length_bp: 149
variable_sites: 6
pi: 0.027
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-psbA_p1	TCTGACTTTCGCGAGACATG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.0	57.9	242-243 bp	1.0	81.6
rps19-psbA_p2	TCTGACTTTCGCGAGACATG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.0	57.9	245-246 bp	1.0	81.5
rps19-psbA_p3	CCTACTCTGACTTTCGCGAG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	57.8	57.9	247-248 bp	1.0	80.3
rps19-psbA_p4	CCTACTCTGACTTTCGCGAG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	57.8	57.9	250-251 bp	1.0	80.2
rps19-psbA_p5	CTCTGACTTTCGCGAGACAT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.7	57.9	243-244 bp	1.0	79.6