Hotspot

rpl2-trnH-GUG

IRb · 41406-41448 · 43 bp

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Pi 0.031

MarkerSeek score 71.00

Primer available yes

Label rpl2-trnH-GUG

Feature Metadata

label_name: rpl2-trnH-GUG
feature_type: igs
region: IRb
length_bp: 43
variable_sites: 2
pi: 0.031
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnH-GUG_p1	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.0	58.1	193 bp	1.0	79.6
rpl2-trnH-GUG_p2	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	50.0	50.0	58.0	57.9	179 bp	1.0	79.6
rpl2-trnH-GUG_p3	GTTATCCTGCGCTTGGAAGA	GGATCAAGGCAGTGGATTGT	50.0	50.0	58.0	57.9	178 bp	1.0	79.6
rpl2-trnH-GUG_p4	GTTATCCTGCGCTTGGAAGA	GTGGATCAAGGCAGTGGATT	50.0	50.0	58.0	57.9	180 bp	1.0	79.6
rpl2-trnH-GUG_p5	CACAACCCCTTGGGGTTATC	CGGATGTAGCCAAGTGGATC	55.0	55.0	58.2	58.1	207 bp	1.0	78.5