Hotspot

rps15-ycf1

SSC · 124358-124739 · 382 bp

Back to Cathetus

Pi 0.118

MarkerSeek score 74.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 382
variable_sites: 45
pi: 0.118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AACACCATGCATTGATGGGT	AAGGGCGAAATTTGTCTGGA	45.0	45.0	58.1	57.7	1434 bp	0.5	57.2
rps15-ycf1_p2	CCAACGGGTTACATAAGGCA	AAGGGCGAAATTTGTCTGGA	50.0	45.0	57.9	57.7	1569 bp	0.5	56.8
rps15-ycf1_p3	TAACATCCTCACCGTCGAGA	AAGGGCGAAATTTGTCTGGA	50.0	45.0	57.9	57.7	1471 bp	0.5	56.8
rps15-ycf1_p4	TGGGGGCCCATATTGACTAT	AAGGGCGAAATTTGTCTGGA	50.0	45.0	57.9	57.7	1412 bp	0.5	56.5
rps15-ycf1_p5	CACCGTCGAGAGTAACGATG	AAGGGCGAAATTTGTCTGGA	55.0	45.0	58.2	57.7	1462 bp	0.5	56.2