Hotspot

rpl32-trnL-UAG

SSC · 114535-115314 · 780 bp

Back to Cathetus

Pi 0.077
MarkerSeek score 79.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
780
variable_sites
51
pi
0.077
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CAAAAGGCTTTTTGTACGACA AGCGTGTCTACCGATTTCAC 38.1 50.0 55.6 58.0 835 bp 0.5 32.5
rpl32-trnL-UAG_p2 CAAAAGGCTTTTTGTACGACA CAGCGTGTCTACCGATTTCA 38.1 50.0 55.6 58.0 836 bp 0.5 32.4
rpl32-trnL-UAG_p3 CAAAAGGCTTTTTGTACGACA TCCTAAGAGCAGCGTGTCTA 38.1 50.0 55.6 57.9 845 bp 0.5 31.5
rpl32-trnL-UAG_p4 CAAAAGGCTTTTTGTACGACA CCTAAGAGCAGCGTGTCTAC 38.1 55.0 55.6 57.8 844 bp 0.5 30.9
rpl32-trnL-UAG_p5 CAAAAGGCTTTTTGTACGACA CTAAGAGCAGCGTGTCTACC 38.1 55.0 55.6 57.8 843 bp 0.5 30.9