Hotspot

psaA-ycf3

LSC · 43233-43932 · 700 bp

Back to Cathetus

Pi 0.0433

MarkerSeek score 79.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 700
variable_sites: 30
pi: 0.0433
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	GCGCTTACCCCTGGAAATTA	50.0	50.0	57.9	58.0	846 bp	1.0	82.2
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TATAGCGCTTACCCCTGGAA	50.0	50.0	57.9	57.9	850 bp	1.0	81.8
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	CTATAGCGCTTACCCCTGGA	50.0	55.0	57.9	58.4	851 bp	1.0	79.6
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	GCGCTTACCCCTGGAAATTA	50.0	50.0	58.5	58.0	799 bp	1.0	79.4
psaA-ycf3_p5	TTGAGAAATGACCGGGTCTG	GCGCTTACCCCTGGAAATTA	50.0	50.0	57.5	58.0	866 bp	1.0	79.3