Hotspot

trnM-rps14

LSC · 38165-38329 · 165 bp

Back to Cathetus

Pi 0.0446

MarkerSeek score 77.60

Primer available yes

Label trnM-rps14

Feature Metadata

label_name: trnM-rps14
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 7
pi: 0.0446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-rps14_p1	AAGGTTATGAGCCTTGCGAG	CCGAGAGCTAACTACCGAGA	50.0	55.0	58.0	58.0	299-307 bp	1.0	83.3
trnM-rps14_p2	AAGGTTATGAGCCTTGCGAG	GCTATCCGGACACGTACTTC	50.0	55.0	58.0	57.9	273-281 bp	1.0	83.2
trnM-rps14_p3	TTTGAACCCGTGACCTCAAG	CCGAGAGCTAACTACCGAGA	50.0	55.0	58.0	58.0	316-324 bp	1.0	83.1
trnM-rps14_p4	TTTGAACCCGTGACCTCAAG	GCTATCCGGACACGTACTTC	50.0	55.0	58.0	57.9	290-298 bp	1.0	82.8
trnM-rps14_p5	GATTTGAACCCGTGACCTCA	CCGAGAGCTAACTACCGAGA	50.0	55.0	57.8	58.0	318-326 bp	1.0	81.9