Hotspot

rps16-trnQ-UUG

LSC · 6900-7829 · 930 bp

Back to Cathetus

Pi 0.233

MarkerSeek score 74.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 930
variable_sites: 121
pi: 0.233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.56
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TGCCCCCGAAAAAGAAATCA	CTTACAGCAGCTTGCCAAAC	45.0	50.0	58.0	57.9	1776 bp	0.5	59.5
rps16-trnQ-UUG_p2	GTCTTTCAAGTCGCACGTTG	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	1592 bp	0.5	59.5
rps16-trnQ-UUG_p3	AGAATCCACAACCGATCGTG	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	1611 bp	0.5	59.5
rps16-trnQ-UUG_p4	TGCCCCCGAAAAAGAAATCA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	1194 bp	0.5	59.4
rps16-trnQ-UUG_p5	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1010 bp	0.5	59.3