Hotspot

trnK-UUU-rps16

LSC · 4488-5736 · 1249 bp

Back to Cathetus

Pi 0.0592
MarkerSeek score 77.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1249
variable_sites
67
pi
0.0592
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGGGTATGGACGAGTTTGTT CGCTTTAACCACACGCAATT 50.0 45.0 57.9 58.0 1320-1368 bp 1.0 87.2
trnK-UUU-rps16_p2 CGGGTATGGACGAGTTTGTT CCCAATGAGCCGTTTATCGA 50.0 50.0 57.9 58.1 1526-1574 bp 1.0 87.1
trnK-UUU-rps16_p3 CGGGTATGGACGAGTTTGTT TCCCAATGAGCCGTTTATCG 50.0 50.0 57.9 58.1 1527-1575 bp 1.0 87.1
trnK-UUU-rps16_p4 CGGGTATGGACGAGTTTGTT GCCGTACGAGGAGAAAACTT 50.0 50.0 57.9 57.9 1586-1636 bp 1.0 86.8
trnK-UUU-rps16_p5 CATCTCCCCCTTGTAGTGGA CGCTTTAACCACACGCAATT 55.0 45.0 58.1 58.0 1705-1754 bp 1.0 86.6