Hotspot

trnH-GUG-psbA

LSC · 104-530 · 427 bp

Back to Cathetus

Pi 0.132

MarkerSeek score 80.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 427
variable_sites: 52
pi: 0.132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	543-582 bp	1.0	90.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	557-596 bp	1.0	90.4
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	556-595 bp	1.0	90.4
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	558-597 bp	1.0	90.4
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	55.0	50.0	58.1	58.0	879-918 bp	1.0	86.7