Hotspot

psaC-ndhE

SSC · 107386-107891 · 506 bp

Back to Catapodium

Pi 0.00132

MarkerSeek score 58.80

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 506
variable_sites: 1
pi: 0.00132
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GCTTTACATCCATCCCAGGG	TTTTGTGATAGCCCTTGCGG	55.0	50.0	58.0	58.8	723-728 bp	1.0	73.9
psaC-ndhE_p2	GGCTTTACATCCATCCCAGG	TTTTGTGATAGCCCTTGCGG	55.0	50.0	58.0	58.8	724-729 bp	1.0	73.9
psaC-ndhE_p3	GTTGGACAAGCTCGTACACA	TTTTGTGATAGCCCTTGCGG	50.0	50.0	58.1	58.8	684-689 bp	1.0	73.0
psaC-ndhE_p4	TGTTGGACAAGCTCGTACAC	TTTTGTGATAGCCCTTGCGG	50.0	50.0	58.1	58.8	685-690 bp	1.0	73.0
psaC-ndhE_p5	CTGTTGGACAAGCTCGTACA	TTTTGTGATAGCCCTTGCGG	50.0	50.0	57.9	58.8	686-691 bp	1.0	72.9