Hotspot

rpl32-trnL-UAG

SSC · 103485-104202 · 718 bp

Back to Catapodium

Pi 0.00464

MarkerSeek score 49.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 718
variable_sites: 5
pi: 0.00464
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ATTTTTCAGCGGCAATGAGC	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.0	58.0	848 bp	1.0	80.4
rpl32-trnL-UAG_p2	ATTTTTCAGCGGCAATGAGC	GGATTCGAACCGAGATGCTT	45.0	50.0	58.0	58.1	851 bp	1.0	80.0
rpl32-trnL-UAG_p3	ATTTTTCAGCGGCAATGAGC	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	821 bp	1.0	79.5
rpl32-trnL-UAG_p4	ATTTTTCAGCGGCAATGAGC	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.0	57.8	814 bp	1.0	79.0
rpl32-trnL-UAG_p5	ATTTTTCAGCGGCAATGAGC	GCTTGAGCACTGCTTCCTAA	45.0	50.0	58.0	58.2	835 bp	1.0	79.0