Hotspot

rpl20-rps12

LSC · 64874-65575 · 702 bp

Back to Catapodium

Pi 0.000951
MarkerSeek score 59.50
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
702
variable_sites
1
pi
0.000951
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 ACGCATTTTTGTTCGTCGTC TAGAAAAACAGCCGCGCTTA 45.0 45.0 58.0 58.2 816-817 bp 1.0 78.5
rpl20-rps12_p2 ACGCATTTTTGTTCGTCGTC AAAAACAGCCGCGCTTAAAG 45.0 45.0 58.0 58.2 813-814 bp 1.0 78.2
rpl20-rps12_p3 ACGCATTTTTGTTCGTCGTC GAAAAACAGCCGCGCTTAAA 45.0 45.0 58.0 58.2 814-815 bp 1.0 78.2
rpl20-rps12_p4 ACGCATTTTTGTTCGTCGTC GAACATGCGCTAGGGTGTAT 45.0 50.0 58.0 57.8 775-776 bp 1.0 78.2
rpl20-rps12_p5 ACGCATTTTTGTTCGTCGTC TAGAAACGCAAGACAGCCAA 45.0 45.0 58.0 57.8 846-847 bp 1.0 78.1