Hotspot

rbcL-psaI

LSC · 54587-55898 · 1312 bp

Back to Catapodium

Pi 0.00508

MarkerSeek score 62.00

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1312
variable_sites: 10
pi: 0.00508
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CGCAGCTTGTGAAGTATGGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	1449 bp	1.0	78.1
rbcL-psaI_p2	AGTTCGAGCCGGTAGATACT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.7	58.0	1414 bp	1.0	77.6
rbcL-psaI_p3	CGCAGCTTGTGAAGTATGGA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.3	57.9	1455 bp	1.0	77.5
rbcL-psaI_p4	GGCGATCAAATTCGAGTTCG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.6	58.0	1428 bp	1.0	77.5
rbcL-psaI_p5	TGGAAGGCGATCAAATTCGA	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.6	58.0	1433 bp	1.0	77.2