Hotspot

trnL-UAA-trnF-GAA

LSC · 45850-46212 · 363 bp

Back to Catapodium

Pi 0.00187

MarkerSeek score 59.00

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 363
variable_sites: 1
pi: 0.00187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	GTTGTGAGGGTTCAAGTCCC	TTTGAACTGGTGACACGAGG	55.0	50.0	58.4	58.1	438-452 bp	1.0	79.1
trnL-UAA-trnF-GAA_p2	GTTGTGAGGGTTCAAGTCCC	CTGCCAGGAACCAGATTTGA	55.0	50.0	58.4	57.8	453-467 bp	1.0	77.8
trnL-UAA-trnF-GAA_p3	GTTGTGAGGGTTCAAGTCCC	TCTGCCAGGAACCAGATTTG	55.0	50.0	58.4	57.8	454-468 bp	1.0	77.8
trnL-UAA-trnF-GAA_p4	GTTGTGAGGGTTCAAGTCCC	TTTTCTCTGCCAGGAACCAG	55.0	50.0	58.4	57.7	459-473 bp	1.0	77.2
trnL-UAA-trnF-GAA_p5	AGTTGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.4	58.1	439-453 bp	1.0	77.2