Hotspot

petN-trnC-GCA

LSC · 15891-16774 · 884 bp

Back to Catapodium

Pi 0.000756

MarkerSeek score 59.40

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 884
variable_sites: 1
pi: 0.000756
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	931-933 bp	1.0	70.6
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	50.0	55.0	58.2	59.1	933-935 bp	1.0	70.2
petN-trnC-GCA_p3	CCTTAGAGTCCACTCCTCCC	AGTTCAAATCTGGGTGCCG	60.0	52.6	58.2	58.1	996-998 bp	1.0	70.1
petN-trnC-GCA_p4	CTTAGAGTCCACTCCTCCCC	AGTTCAAATCTGGGTGCCG	60.0	52.6	58.2	58.1	995-997 bp	1.0	70.1
petN-trnC-GCA_p5	CTTAGAGTCCACTCCTCCCC	CCAGTTCAAATCTGGGTGCC	60.0	55.0	58.2	59.1	997-999 bp	1.0	69.6