primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TGGACGAATTTGTTGCTCCA	TCAACCGACAAGAACCGTTT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.052738	3956	4512	719-719	1.000000	79.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TGGACGAATTTGTTGCTCCA	CTCAACCGACAAGAACCGTT	20	20	45.0	50.0	58.0	58.4	0.0	0.0	0.447152	3956	4512	720-720	1.000000	76.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AGTCGCACTTAAAAGCCGAG	TCAACCGACAAGAACCGTTT	20	20	50.0	45.0	58.6	58.0	6.4	0.0	0.597541	3956	4512	678-678	1.000000	75.5
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CTGGACGAATTTGTTGCTCC	TCAACCGACAAGAACCGTTT	20	20	50.0	45.0	57.4	58.0	0.0	0.0	0.646017	3956	4512	720-720	1.000000	75.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCGCACTTAAAAGCCGAGTA	TCAACCGACAAGAACCGTTT	20	20	45.0	45.0	57.3	58.0	3.0	0.0	0.755693	3956	4512	676-676	1.000000	74.2
petN-trnC-GCA_p1	petN-trnC-GCA	1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	20	19	50.0	52.6	58.2	58.1	0.0	0.0	1.204402	15891	16774	931-933	1.000000	70.6
petN-trnC-GCA_p2	petN-trnC-GCA	2	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	20	20	50.0	55.0	58.2	59.1	0.0	4.3	1.260665	15891	16774	933-935	1.000000	70.2
petN-trnC-GCA_p3	petN-trnC-GCA	3	CCTTAGAGTCCACTCCTCCC	AGTTCAAATCTGGGTGCCG	20	19	60.0	52.6	58.2	58.1	3.0	0.0	1.269982	15891	16774	996-998	1.000000	70.1
petN-trnC-GCA_p4	petN-trnC-GCA	4	CTTAGAGTCCACTCCTCCCC	AGTTCAAATCTGGGTGCCG	20	19	60.0	52.6	58.2	58.1	3.0	0.0	1.269982	15891	16774	995-997	1.000000	70.1
petN-trnC-GCA_p5	petN-trnC-GCA	5	CTTAGAGTCCACTCCTCCCC	CCAGTTCAAATCTGGGTGCC	20	20	60.0	55.0	58.2	59.1	3.0	4.3	1.326246	15891	16774	997-999	1.000000	69.6
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	20	20	50.0	50.0	58.1	58.0	14.6	0.0	0.109519	16846	18083	1266-1307	1.000000	81.8
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	20	20	50.0	50.0	58.1	57.8	14.6	0.0	0.250044	16846	18083	1273-1314	1.000000	80.6
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	AAAGGATTTGCAGTCCCCTG	GTTCTTCTGCCAGGGCTTTA	20	20	50.0	50.0	58.1	57.8	14.6	12.2	0.271505	16846	18083	1343-1384	1.000000	80.3
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	AAAGGATTTGCAGTCCCCTG	TGTCAAGTTCTTCTGCCAGG	20	20	50.0	50.0	58.1	57.7	14.6	0.0	0.331976	16846	18083	1349-1390	1.000000	79.8
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	16846	18083	1280-1321	1.000000	79.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	44404	45220	918-919	1.000000	79.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	44404	45220	910-911	1.000000	79.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140570	44404	45220	888-889	1.000000	79.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.272624	44404	45220	908-909	1.000000	78.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.303758	44404	45220	917-918	1.000000	77.7
trnL-UAA_p1	trnL-UAA	1	CGAACTGCTAGACTACGTCG	AGTTAGGGAATAAAGGAGGGT	20	21	55.0	42.9	57.9	55.3	0.0	0.0	3.737239	45221	45849	754-754	1.000000	50.3
trnL-UAA_p2	trnL-UAA	2	CGAACTGCTAGACTACGTCG	AAGTTAGGGAATAAAGGAGGGT	20	22	55.0	40.9	57.9	56.1	0.0	0.0	3.941300	45221	45849	755-755	1.000000	48.7
trnL-UAA_p3	trnL-UAA	3	CGAACTGCTAGACTACGTCG	AGTTAGGGAATAAAGGAGGGTT	20	22	55.0	40.9	57.9	56.1	0.0	0.0	3.941300	45221	45849	754-754	1.000000	48.7
trnL-UAA_p4	trnL-UAA	4	TGCTAGACTACGTCGAGTGA	AGTTAGGGAATAAAGGAGGGT	20	21	50.0	42.9	57.6	55.3	0.0	0.0	4.038036	45221	45849	749-749	1.000000	47.9
trnL-UAA_p5	trnL-UAA	5	ACTGCTAGACTACGTCGAGT	AGTTAGGGAATAAAGGAGGGT	20	21	50.0	42.9	57.6	55.3	0.0	0.0	4.046231	45221	45849	751-751	1.000000	47.8
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	GTTGTGAGGGTTCAAGTCCC	TTTGAACTGGTGACACGAGG	20	20	55.0	50.0	58.4	58.1	0.0	20.1	0.446143	45850	46212	438-452	1.000000	79.1
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	GTTGTGAGGGTTCAAGTCCC	CTGCCAGGAACCAGATTTGA	20	20	55.0	50.0	58.4	57.8	0.0	0.0	0.599270	45850	46212	453-467	1.000000	77.8
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	GTTGTGAGGGTTCAAGTCCC	TCTGCCAGGAACCAGATTTG	20	20	55.0	50.0	58.4	57.8	0.0	0.0	0.599270	45850	46212	454-468	1.000000	77.8
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	GTTGTGAGGGTTCAAGTCCC	TTTTCTCTGCCAGGAACCAG	20	20	55.0	50.0	58.4	57.7	0.0	0.0	0.670979	45850	46212	459-473	1.000000	77.2
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	AGTTGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.4	58.1	0.0	20.1	0.692212	45850	46212	439-453	1.000000	77.2
trnV-UAC_p1	trnV-UAC	1	TTCGATGAATGAGCCTGTGG	ACCGCTTCATTATGGGTCTG	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.360483	49341	50020	864-864	1.000000	77.3
trnV-UAC_p2	trnV-UAC	2	TTCGATGAATGAGCCTGTGG	CCGCTTCATTATGGGTCTGT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.360483	49341	50020	863-863	1.000000	77.3
trnV-UAC_p3	trnV-UAC	3	TGAGCCTGTGGTAATGCTTT	ACCGCTTCATTATGGGTCTG	20	20	45.0	50.0	57.4	57.7	0.0	0.0	0.916898	49341	50020	855-855	1.000000	72.9
trnV-UAC_p4	trnV-UAC	4	TGAGCCTGTGGTAATGCTTT	CCGCTTCATTATGGGTCTGT	20	20	45.0	50.0	57.4	57.7	0.0	0.0	0.916898	49341	50020	854-854	1.000000	72.9
trnV-UAC_p5	trnV-UAC	5	CGATGAATGAGCCTGTGGTA	CCGCTTCATTATGGGTCTGT	20	20	50.0	50.0	57.4	57.7	0.0	0.0	0.934320	49341	50020	861-861	1.000000	72.7
rbcL-psaI_p1	rbcL-psaI	1	CGCAGCTTGTGAAGTATGGA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.3	58.0	0.0	26.9	0.301363	54587	55898	1449-1449	1.000000	78.1
rbcL-psaI_p2	rbcL-psaI	2	AGTTCGAGCCGGTAGATACT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.7	58.0	1.9	26.9	0.372128	54587	55898	1414-1414	1.000000	77.6
rbcL-psaI_p3	rbcL-psaI	3	CGCAGCTTGTGAAGTATGGA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.3	57.9	0.0	26.8	0.377897	54587	55898	1455-1455	1.000000	77.5
rbcL-psaI_p4	rbcL-psaI	4	GGCGATCAAATTCGAGTTCG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.6	58.0	0.0	26.9	0.380261	54587	55898	1428-1428	1.000000	77.5
rbcL-psaI_p5	rbcL-psaI	5	TGGAAGGCGATCAAATTCGA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.6	58.0	0.0	26.9	0.421933	54587	55898	1433-1433	1.000000	77.2
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	ACGGTTCATATTCTGGATTGGG	20	22	50.0	45.5	58.0	58.2	0.0	0.0	2.217830	59096	59943	1002-1002	1.000000	62.7
petA-psbJ_p2	petA-psbJ	2	ATCCATTACGTGTCCAAGGC	CGGTTCATATTCTGGATTGGGT	20	22	50.0	45.5	58.0	58.2	0.0	0.0	2.217830	59096	59943	1001-1001	1.000000	62.7
petA-psbJ_p3	petA-psbJ	3	TTTGTTTTTCTTCGCGTCCG	ACGGTTCATATTCTGGATTGGG	20	22	45.0	45.5	57.9	58.2	5.8	0.0	2.273415	59096	59943	980-980	1.000000	62.3
petA-psbJ_p4	petA-psbJ	4	TTTGTTTTTCTTCGCGTCCG	CGGTTCATATTCTGGATTGGGT	20	22	45.0	45.5	57.9	58.2	5.8	0.0	2.273415	59096	59943	979-979	1.000000	62.3
petA-psbJ_p5	petA-psbJ	5	CTTCGCGTCCGTTATTTTGG	CGGTTCATATTCTGGATTGGGT	20	22	50.0	45.5	57.8	58.2	5.8	0.0	2.333123	59096	59943	970-970	1.000000	61.8
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	AAAACAAACGCGCTACCAAG	ACGGGTGCTACAGAGAGATA	20	20	45.0	50.0	57.9	57.3	5.7	0.0	0.844845	62427	62788	435-435	1.000000	73.6
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CAAAACAAACGCGCTACCAA	ACGGGTGCTACAGAGAGATA	20	20	45.0	50.0	58.2	57.3	5.7	0.0	0.902103	62427	62788	436-436	1.000000	73.2
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	TTGTACCCAAAACAAACGCG	ACGGGTGCTACAGAGAGATA	20	20	45.0	50.0	57.8	57.3	3.5	0.0	0.921259	62427	62788	443-443	1.000000	73.0
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	AAAACAAACGCGCTACCAAG	CACGGGTGCTACAGAGAGAT	20	20	45.0	55.0	57.9	58.9	5.7	0.0	1.006796	62427	62788	436-436	1.000000	72.3
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	CAAAACAAACGCGCTACCAA	CACGGGTGCTACAGAGAGAT	20	20	45.0	55.0	58.2	58.9	5.7	0.0	1.064054	62427	62788	437-437	1.000000	71.9
rpl20-rps12_p1	rpl20-rps12	1	ACGCATTTTTGTTCGTCGTC	TAGAAAAACAGCCGCGCTTA	20	20	45.0	45.0	58.0	58.2	0.0	5.6	0.215002	64874	65575	816-817	1.000000	78.5
rpl20-rps12_p2	rpl20-rps12	2	ACGCATTTTTGTTCGTCGTC	AAAAACAGCCGCGCTTAAAG	20	20	45.0	45.0	58.0	58.2	0.0	5.6	0.247217	64874	65575	813-814	1.000000	78.2
rpl20-rps12_p3	rpl20-rps12	3	ACGCATTTTTGTTCGTCGTC	GAAAAACAGCCGCGCTTAAA	20	20	45.0	45.0	58.0	58.2	0.0	5.6	0.252080	64874	65575	814-815	1.000000	78.2
rpl20-rps12_p4	rpl20-rps12	4	ACGCATTTTTGTTCGTCGTC	GAACATGCGCTAGGGTGTAT	20	20	45.0	50.0	58.0	57.8	0.0	9.7	0.253779	64874	65575	775-776	1.000000	78.2
rpl20-rps12_p5	rpl20-rps12	5	ACGCATTTTTGTTCGTCGTC	TAGAAACGCAAGACAGCCAA	20	20	45.0	45.0	58.0	57.8	0.0	0.0	0.261566	64874	65575	846-847	1.000000	78.1
rpoA_p1	rpoA	1	GTTCCTTTGGGGGATGGATC	CCATTGCTAAAAGCGGTGTG	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.091998	72479	73504	1912-1912	1.000000	79.4
rpoA_p2	rpoA	2	GTTCCTTTGGGGGATGGATC	ATGGTAAAAGGCGCTGGTAG	20	20	55.0	50.0	57.9	58.0	0.0	7.6	0.111639	72479	73504	1955-1955	1.000000	79.2
rpoA_p3	rpoA	3	GTTCCTTTGGGGGATGGATC	GTGGAAGAGATGCCGCATTA	20	20	55.0	50.0	57.9	58.0	0.0	7.4	0.119693	72479	73504	1936-1936	1.000000	79.2
rpoA_p4	rpoA	4	GTTCCTTTGGGGGATGGATC	TGGAAGAGATGCCGCATTAC	20	20	55.0	50.0	57.9	58.0	0.0	7.4	0.119693	72479	73504	1935-1935	1.000000	79.2
rpoA_p5	rpoA	5	GTTCCTTTGGGGGATGGATC	GATGTAACACCTATGCCGCA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.125148	72479	73504	1874-1874	1.000000	79.1
ndhF-rpl32_p1	ndhF-rpl32	1	GGAAGTGGGAGAAGAGGGAT	TGCTAAAGAATAAGCGTGTACT	20	22	55.0	36.4	58.1	55.6	0.0	0.0	4.531268	102442	103304	1018-1018	1.000000	44.0
ndhF-rpl32_p2	ndhF-rpl32	2	GGCCAAACCCCATTGACATA	TGCTAAAGAATAAGCGTGTACT	20	22	50.0	36.4	58.1	55.6	0.0	0.0	4.558388	102442	103304	1044-1044	1.000000	43.8
ndhF-rpl32_p3	ndhF-rpl32	3	AAAAGGCCAAACCCCATTGA	TGCTAAAGAATAAGCGTGTACT	20	22	45.0	36.4	58.2	55.6	5.0	0.0	4.604588	102442	103304	1048-1048	1.000000	43.4
ndhF-rpl32_p4	ndhF-rpl32	4	GGAAGTGGGAGAAGAGGGAT	TTGCTAAAGAATAAGCGTGTACT	20	23	55.0	34.8	58.1	56.3	0.0	0.0	4.811693	102442	103304	1019-1019	1.000000	41.7
ndhF-rpl32_p5	ndhF-rpl32	5	GGCCAAACCCCATTGACATA	TTGCTAAAGAATAAGCGTGTACT	20	23	50.0	34.8	58.1	56.3	0.0	0.0	4.838814	102442	103304	1045-1045	1.000000	41.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	ATTTTTCAGCGGCAATGAGC	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	58.0	58.0	1.4	0.0	0.004138	103485	104202	848-848	1.000000	80.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	ATTTTTCAGCGGCAATGAGC	GGATTCGAACCGAGATGCTT	20	20	45.0	50.0	58.0	58.1	1.4	0.0	0.059104	103485	104202	851-851	1.000000	80.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	ATTTTTCAGCGGCAATGAGC	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.0	57.9	1.4	0.0	0.124706	103485	104202	821-821	1.000000	79.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	ATTTTTCAGCGGCAATGAGC	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.0	57.8	1.4	0.0	0.185433	103485	104202	814-814	1.000000	79.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ATTTTTCAGCGGCAATGAGC	GCTTGAGCACTGCTTCCTAA	20	20	45.0	50.0	58.0	58.2	1.4	4.7	0.186916	103485	104202	835-835	1.000000	79.0
psaC-ndhE_p1	psaC-ndhE	1	GCTTTACATCCATCCCAGGG	TTTTGTGATAGCCCTTGCGG	20	20	55.0	50.0	58.0	58.8	0.0	0.0	0.840074	107386	107891	723-728	1.000000	73.9
psaC-ndhE_p2	psaC-ndhE	2	GGCTTTACATCCATCCCAGG	TTTTGTGATAGCCCTTGCGG	20	20	55.0	50.0	58.0	58.8	0.0	0.0	0.840074	107386	107891	724-729	1.000000	73.9
psaC-ndhE_p3	psaC-ndhE	3	GTTGGACAAGCTCGTACACA	TTTTGTGATAGCCCTTGCGG	20	20	50.0	50.0	58.1	58.8	0.0	0.0	0.966281	107386	107891	684-689	1.000000	73.0
psaC-ndhE_p4	psaC-ndhE	4	TGTTGGACAAGCTCGTACAC	TTTTGTGATAGCCCTTGCGG	20	20	50.0	50.0	58.1	58.8	0.0	0.0	0.966281	107386	107891	685-690	1.000000	73.0
psaC-ndhE_p5	psaC-ndhE	5	CTGTTGGACAAGCTCGTACA	TTTTGTGATAGCCCTTGCGG	20	20	50.0	50.0	57.9	58.8	0.0	0.0	0.975872	107386	107891	686-691	1.000000	72.9
ndhA_p1	ndhA	1	ACCCAGTTACCATAGGGAACA	TATGGGGGAAGTTGACCGTT	21	20	47.6	50.0	58.1	58.6	13.3	0.0	1.711824	109711	111825	2257-2257	1.000000	66.4
ndhA_p2	ndhA	2	ACCCAGTTACCATAGGGAACA	TTATGGGGGAAGTTGACCGT	21	20	47.6	50.0	58.1	58.6	13.3	0.0	1.711824	109711	111825	2258-2258	1.000000	66.4
ndhA_p3	ndhA	3	ACCCAGTTACCATAGGGAACA	ATTATGGGGGAAGTTGACCG	21	20	47.6	50.0	58.1	57.3	13.3	0.0	1.805120	109711	111825	2259-2259	1.000000	65.6
ndhA_p4	ndhA	4	ACCCAGTTACCATAGGGAACA	TATGGGGGAAGTTGACCGT	21	19	47.6	52.6	58.1	57.9	13.3	0.0	2.168180	109711	111825	2257-2257	1.000000	62.7
ndhA_p5	ndhA	5	CCCAGTTACCATAGGGAACA	TATGGGGGAAGTTGACCGTT	20	20	50.0	50.0	56.2	58.6	13.3	0.0	2.422386	109711	111825	2256-2256	1.000000	60.7