Hotspot

psaC

SSC · 122070-122315 · 246 bp

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Pi 0.00163

MarkerSeek score 25.40

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 1
pi: 0.00163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TCAAGTAGAGTTTTATGGAACGT	CGTTCAAAAATGTATAGATCCA	34.8	31.8	55.6	52.3	292 bp	1.0	40.3
psaC_p2	TTCAAGTAGAGTTTTATGGAACGT	CGTTCAAAAATGTATAGATCCA	33.3	31.8	56.3	52.3	293 bp	1.0	40.3
psaC_p3	CAAGTAGAGTTTTATGGAACGT	CGTTCAAAAATGTATAGATCCA	36.4	31.8	54.2	52.3	291 bp	1.0	40.3
psaC_p4	TCAAGTAGAGTTTTATGGAACG	CGTTCAAAAATGTATAGATCCA	36.4	31.8	53.9	52.3	292 bp	1.0	40.3
psaC_p5	ATTCAAGTAGAGTTTTATGGAACGT	CGTTCAAAAATGTATAGATCCA	32.0	31.8	56.8	52.3	294 bp	1.0	40.3