Hotspot

ndhH

SSC · 126656-127837 · 1182 bp

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Pi 0.00305

MarkerSeek score 32.20

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 8
pi: 0.00305
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TGAAGAATTCCCAAAGGCCC	GAAAACGTCAACGACTGCTG	50.0	50.0	58.1	58.0	1602 bp	1.0	80.3
ndhH_p2	GCTATGGAGGGACCGATACT	GAAAACGTCAACGACTGCTG	55.0	50.0	58.1	58.0	1695 bp	1.0	80.2
ndhH_p3	TGAAGAATTCCCAAAGGCCC	TTGGGAAAACGTCAACGACT	50.0	45.0	58.1	58.0	1606 bp	1.0	80.1
ndhH_p4	TGAAGAATTCCCAAAGGCCC	TGCATGGAAGAATGACTCGG	50.0	50.0	58.1	58.0	1434 bp	1.0	79.8
ndhH_p5	CTGCTATGGAGGGACCGATA	GAAAACGTCAACGACTGCTG	55.0	50.0	58.1	58.0	1697 bp	0.6	64.1