Hotspot

rpl32-trnL-UAC

SSC · 118195-119078 · 884 bp

Back to Catalpa

Pi 0.0147

MarkerSeek score 55.40

Primer available yes

Label rpl32-trnL-UAC

Feature Metadata

label_name: rpl32-trnL-UAC
feature_type: igs
region: SSC
length_bp: 884
variable_sites: 26
pi: 0.0147
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAC_p1	GGGAAATCTCTTTCTACCGGG	TCGAACCGAGATGCTTTAGC	52.4	50.0	57.8	58.1	1008-1019 bp	1.0	73.3
rpl32-trnL-UAC_p2	GGGAAATCTCTTTCTACCGGG	TCCTAAGAGCAGCGTGTCTA	52.4	50.0	57.8	57.9	982-993 bp	1.0	73.0
rpl32-trnL-UAC_p3	GGGAAATCTCTTTCTACCGGG	AGCAGCGTGTCTACCAATTT	52.4	45.0	57.8	57.8	975-986 bp	1.0	72.5
rpl32-trnL-UAC_p4	GGGAAATCTCTTTCTACCGGG	CCTAAGAGCAGCGTGTCTAC	52.4	55.0	57.8	57.8	981-992 bp	1.0	72.4
rpl32-trnL-UAC_p5	GGGAAATCTCTTTCTACCGGG	CTAAGAGCAGCGTGTCTACC	52.4	55.0	57.8	57.8	980-991 bp	1.0	72.4