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psaJ-rpl33

LSC · 68234-68700 · 467 bp

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Pi 0.00642

MarkerSeek score 47.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 467
variable_sites: 6
pi: 0.00642
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATGCGTTGACATTCCCCTTT	TTACTCGCGCATCTTTCCTC	45.0	50.0	58.1	58.1	527-528 bp	1.0	80.6
psaJ-rpl33_p2	ATGCGTTGACATTCCCCTTT	GCCCGTTGACACCTTCTTAA	45.0	50.0	58.1	58.1	586-587 bp	1.0	80.1
psaJ-rpl33_p3	ATGCGTTGACATTCCCCTTT	CAAAATCACCGTTACTCGCG	45.0	50.0	58.1	57.9	538-539 bp	1.0	80.1
psaJ-rpl33_p4	ATGCGTTGACATTCCCCTTT	GCATCTTTCCTCTTGGCCAT	45.0	50.0	58.1	58.2	519-520 bp	1.0	79.4
psaJ-rpl33_p5	TGCGTTGACATTCCCCTTTT	TTACTCGCGCATCTTTCCTC	45.0	50.0	58.3	58.1	526-527 bp	1.0	78.8