Hotspot

ndhC-trnV-UAC

LSC · 51125-52248 · 1124 bp

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Pi 0.00552
MarkerSeek score 47.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1124
variable_sites
14
pi
0.00552
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAATCCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1255-1275 bp 1.0 78.7
ndhC-trnV-UAC_p2 TTCTCCGGCCCTTTCCTAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 1276-1296 bp 1.0 76.1
ndhC-trnV-UAC_p3 GGGGCTAAAATCCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1254-1274 bp 1.0 76.0
ndhC-trnV-UAC_p4 GGGGCTAAAATCCCGGAAAT GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 1243-1263 bp 1.0 74.8
ndhC-trnV-UAC_p5 CCCTTTCCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.6 57.8 1268-1288 bp 1.0 74.8