Hotspot

trnF-GAA-ndhJ

LSC · 48774-49453 · 680 bp

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Pi 0.00971

MarkerSeek score 44.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 680
variable_sites: 12
pi: 0.00971
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGATGGGATGCTACATTGGG	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.1	58.1	842-846 bp	1.0	80.1
trnF-GAA-ndhJ_p2	ATGGTCAGGATAGCTCAGCT	AGTTGGATAGGATGGCCCTT	50.0	50.0	57.9	58.1	821-825 bp	1.0	80.1
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	803-807 bp	1.0	79.8
trnF-GAA-ndhJ_p4	TGGGAATGGTCAGGATAGCT	AGTTGGATAGGATGGCCCTT	50.0	50.0	57.8	58.1	826-830 bp	1.0	79.3
trnF-GAA-ndhJ_p5	ACTGAAAATCCTCGTGTCGC	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.6	58.1	788-792 bp	1.0	76.4