Hotspot

rpoB-trnC-GCA

LSC · 27319-28472 · 1154 bp

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Pi 0.00607

MarkerSeek score 38.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1154
variable_sites: 15
pi: 0.00607
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CATTGCCTCATTTGCATCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1243-1245 bp	1.0	78.4
rpoB-trnC-GCA_p2	CATTGCCTCATTTGCATCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.8	58.4	1225-1227 bp	1.0	76.3
rpoB-trnC-GCA_p3	ATTGCCTCATTTGCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.9	58.1	1242-1244 bp	1.0	73.3
rpoB-trnC-GCA_p4	CATTGCCTCATTTGCATCCC	TTTGTTGATCAGGCGACACC	50.0	50.0	57.8	58.8	1262-1264 bp	1.0	73.0
rpoB-trnC-GCA_p5	ACATTGCCTCATTTGCATCC	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.0	58.1	1244-1246 bp	1.0	72.5