Hotspot

rps2-rpoC2

LSC · 16713-16925 · 213 bp

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Pi 0.0119

MarkerSeek score 50.50

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 213
variable_sites: 4
pi: 0.0119
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCTCCAGCATCTCTTCCAAG	TTCGAGGGGGAAATGAGAGA	55.0	50.0	57.7	57.8	372-376 bp	1.0	77.3
rps2-rpoC2_p2	AAATGAACTCCTGCCTCCAG	TTCGAGGGGGAAATGAGAGA	50.0	50.0	57.5	57.8	385-389 bp	1.0	75.8
rps2-rpoC2_p3	CCTCCAGCATCTCTTCCAAG	TCGAGGGGGAAATGAGAGAT	55.0	50.0	57.7	57.5	371-375 bp	1.0	75.5
rps2-rpoC2_p4	CCATGGCCAAAATGAACTCC	TTCGAGGGGGAAATGAGAGA	50.0	50.0	57.3	57.8	394-398 bp	1.0	74.4
rps2-rpoC2_p5	AAATGAACTCCTGCCTCCAG	TCGAGGGGGAAATGAGAGAT	50.0	50.0	57.5	57.5	384-388 bp	1.0	74.0