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trnK-UUU-rps16

LSC · 4238-5121 · 884 bp

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Pi 0.00837

MarkerSeek score 41.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 884
variable_sites: 13
pi: 0.00837
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GTGCTCAACCCACAGGAAC	50.0	57.9	58.2	58.7	999-1008 bp	1.0	67.5
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GTGCTCAACCCACAGGAAC	50.0	57.9	58.2	58.7	998-1007 bp	1.0	67.5
trnK-UUU-rps16_p3	TCTAGCCGCACTTAAAAGCC	GTGCTCAACCCACAGGAAC	50.0	57.9	58.3	58.7	1013-1022 bp	1.0	66.6
trnK-UUU-rps16_p4	AAAGCCGAGTACTCTACCGT	TGCTCAACCCACAGGAACT	50.0	52.6	58.2	58.8	998-1007 bp	1.0	66.6
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	TGCTCAACCCACAGGAACT	50.0	52.6	58.2	58.8	997-1006 bp	1.0	66.6