Hotspot

ycf1

SSC · 126112-131820 · 5709 bp

Back to Casuarina

Pi 0.0014

MarkerSeek score 50.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5709
variable_sites: 12
pi: 0.0014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCCGAATGGTTTGTATTTAGACG	TGGCGAAATACAGTCAATGA	43.5	40.0	58.4	55.1	300 bp	1.0	40.5
ycf1_p2	GCCGAATGGTTTGTATTTAGACG	AGCTGGTTGAGATTAGTACCA	43.5	42.9	58.4	56.1	327 bp	1.0	40.5
ycf1_p3	TGCCGAATGGTTTGTATTTAGAC	TGGCGAAATACAGTCAATGA	39.1	40.0	57.4	55.1	301 bp	1.0	40.5
ycf1_p4	TGCCGAATGGTTTGTATTTAGAC	AGCTGGTTGAGATTAGTACCA	39.1	42.9	57.4	56.1	328 bp	1.0	40.5
ycf1_p5	GCCGAATGGTTTGTATTTAGACG	TGGCGAAATACAGTCAATGAA	43.5	38.1	58.4	55.9	300 bp	1.0	40.5