Hotspot

rpoC2

LSC · 17326-21510 · 4185 bp

Back to Casuarina

Pi 0.00287

MarkerSeek score 42.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4185
variable_sites: 18
pi: 0.00287
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CCTCGCAATGATTGTTTGGG	AAACTGCTCGGGTTTTAGCA	50.0	45.0	57.7	58.0	277 bp	1.0	78.4
rpoC2_p2	CGCAATGATTGTTTGGGTGT	AAACTGCTCGGGTTTTAGCA	45.0	45.0	57.6	58.0	274 bp	1.0	77.2
rpoC2_p3	CCTCGCAATGATTGTTTGGG	CTCTCCGAGGTCGTATCGAT	50.0	55.0	57.7	58.2	250 bp	1.0	77.1
rpoC2_p4	CCTCGCAATGATTGTTTGGG	TCTCCGAGGTCGTATCGATT	50.0	50.0	57.7	57.7	249 bp	1.0	76.7
rpoC2_p5	CCTCGCAATGATTGTTTGGG	GGTTTTAGCAAAAGCCGCTC	50.0	50.0	57.7	58.3	267 bp	1.0	76.2