Hotspot

rpoC1

LSC · 21668-24599 · 2932 bp

Back to Casuarina

Pi 0.00273
MarkerSeek score 45.00
Primer available yes
Label rpoC1

Feature Metadata

label_name
rpoC1
feature_type
gene
region
LSC
length_bp
2932
variable_sites
12
pi
0.00273
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1_p1 ACCCTAATTCATATCGGTGTCA CGGAATGAATCAGAATTTTTCTTCT 40.9 32.0 56.7 56.4 3000 bp 0.333333 13.3
rpoC1_p2 ACCCTAATTCATATCGGTGTCA CGGAATGAATCAGAATTTTTCTTC 40.9 33.3 56.7 55.1 3000 bp 0.333333 13.3
rpoC1_p3 ACCCTAATTCATATCGGTGTCA CGGAATGAATCAGAATTTTTCT 40.9 31.8 56.7 53.1 3000 bp 0.333333 13.3
rpoC1_p4 ACCCTAATTCATATCGGTGTCA CGGAATGAATCAGAATTTTTCTT 40.9 30.4 56.7 53.9 3000 bp 0.333333 13.3
rpoC1_p5 TCATATCGGTGTCATAGAATGA AGCTTAATCGGAATGAATCAGA 36.4 36.4 54.3 55.3 3000 bp 0.333333 13.3