Hotspot

trnR-ACG-trnN-GUU

IRb · 109488-110106 · 619 bp

Back to Casuarina

Pi 0.00325

MarkerSeek score 61.30

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 619
variable_sites: 3
pi: 0.00325
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	757-760 bp	1.0	78.9
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	ACTGACTGGTCGTAGGTTCA	55.0	50.0	57.8	58.0	714-717 bp	1.0	78.9
trnR-ACG-trnN-GUU_p3	GGATACTCCCGGGAACAGAT	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	58.3	58.0	806-809 bp	1.0	78.3
trnR-ACG-trnN-GUU_p4	GGATACTCCCGGGAACAGAT	ACTGACTGGTCGTAGGTTCA	55.0	50.0	58.3	58.0	763-766 bp	1.0	78.3
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	798-801 bp	1.0	78.1