Hotspot

rps18-rpl20

LSC · 70182-70223 · 42 bp

Back to Casuarina

Pi 0.0159

MarkerSeek score 50.10

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 42
variable_sites: 1
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	AATTTGAAGGGGGTGAGTCG	CGTAAAATACTTGCACAAATCGC	50.0	39.1	57.8	57.7	206 bp	1.0	52.0
rps18-rpl20_p2	GGGTGAGTCGACCACTAGAA	CGTAAAATACTTGCACAAATCGC	55.0	39.1	58.5	57.7	196 bp	1.0	50.0
rps18-rpl20_p3	GGTGAGTCGACCACTAGAAC	CGTAAAATACTTGCACAAATCGC	55.0	39.1	57.4	57.7	195 bp	1.0	48.6
rps18-rpl20_p4	ATTTGAAGGGGGTGAGTCGA	CGTAAAATACTTGCACAAATCGC	50.0	39.1	58.6	57.7	205 bp	1.0	48.5
rps18-rpl20_p5	AATTTGAAGGGGGTGAGTCG	AAATACTTGCACAAATCGCT	50.0	35.0	57.8	54.2	202 bp	1.0	48.2