Hotspot

rbcL-accD

LSC · 58878-59609 · 732 bp

Back to Casuarina

Pi 0.00367
MarkerSeek score 49.30
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
732
variable_sites
4
pi
0.00367
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GAGGCTGCTAAATGGAGTCC CAATAGGACCAAGACCAGCC 55.0 55.0 58.0 58.0 911-916 bp 1.0 80.2
rbcL-accD_p2 TTTGAATTCCCGGCAATGGA CAATAGGACCAAGACCAGCC 45.0 55.0 58.1 58.0 851-856 bp 1.0 80.0
rbcL-accD_p3 GAGGCTGCTAAATGGAGTCC ATAGGACCAAGACCAGCCAT 55.0 50.0 58.0 58.1 909-914 bp 1.0 79.8
rbcL-accD_p4 TTTGAATTCCCGGCAATGGA ATAGGACCAAGACCAGCCAT 45.0 50.0 58.1 58.1 849-854 bp 1.0 79.6
rbcL-accD_p5 GAGGCTGCTAAATGGAGTCC GACCAAGACCAGCCATTGAT 55.0 50.0 58.0 57.9 905-910 bp 1.0 79.5