Hotspot

trnF-GAA-ndhJ

LSC · 50699-51477 · 779 bp

Back to Casuarina

Pi 0.00428

MarkerSeek score 49.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 779
variable_sites: 5
pi: 0.00428
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATGCCGGAAAGTTGGATAGG	55.0	50.0	58.0	57.7	929-930 bp	1.0	77.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATGCCGGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	893-894 bp	1.0	77.3
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATGCCGGAAAGTTGGATAGG	55.0	50.0	57.9	57.7	917-918 bp	1.0	76.6
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	GCCGGAAAGTTGGATAGGAT	55.0	50.0	58.0	57.4	927-928 bp	1.0	75.4
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	GCCGGAAAGTTGGATAGGAT	50.0	50.0	58.1	57.4	891-892 bp	1.0	75.0