Hotspot

psbC-trnS-UGA

LSC · 37520-37774 · 255 bp

Back to Casuarina

Pi 0.00523

MarkerSeek score 55.80

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 255
variable_sites: 2
pi: 0.00523
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TTTATGGCATGCAGGAAGGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.2	58.1	432-433 bp	1.0	78.1
psbC-trnS-UGA_p2	TTTATGGCATGCAGGAAGGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.2	58.2	378-379 bp	1.0	76.9
psbC-trnS-UGA_p3	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	404-405 bp	1.0	76.4
psbC-trnS-UGA_p4	TTTATGGCATGCAGGAAGGG	TGGTTGATAGCTCCGGTCTT	50.0	50.0	58.2	58.4	433-434 bp	1.0	75.2
psbC-trnS-UGA_p5	GCTGCAGCAGGATTTGAAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.6	58.2	350-351 bp	1.0	75.2