Hotspot

psbM-trnD-GUC

LSC · 31005-32153 · 1149 bp

Back to Casuarina

Pi 0.0029
MarkerSeek score 58.60
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1149
variable_sites
5
pi
0.0029
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGTGCAGTAGCCATAAGTGC TTCAATTGGTCAGAGCACCG 50.0 50.0 58.0 58.5 1251-1255 bp 1.0 76.3
psbM-trnD-GUC_p2 AGTGCAGTAGCCATAAGTGC GTTCAATTGGTCAGAGCACC 50.0 50.0 58.0 57.0 1252-1256 bp 1.0 72.2
psbM-trnD-GUC_p3 AATGAACAGTGCAGTAGCCA TTCAATTGGTCAGAGCACCG 45.0 50.0 57.4 58.5 1258-1262 bp 1.0 72.0
psbM-trnD-GUC_p4 AGTGCAGTAGCCATAAGTGC TCAATTGGTCAGAGCACCG 50.0 52.6 58.0 57.8 1250-1254 bp 1.0 70.3
psbM-trnD-GUC_p5 GCAGTAGCCATAAGTGCGAG TTCAATTGGTCAGAGCACCG 55.0 50.0 58.8 58.5 1248-1252 bp 1.0 70.3