Hotspot

ycf1

SSC · 130324-136005 · 5682 bp

Back to Castanopsis

Pi 0.00195

MarkerSeek score 57.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5682
variable_sites: 41
pi: 0.00195
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGATGTTGATTTTACTGCAACCT	TATTGAACGGACCCTATCGC	34.8	50.0	57.0	57.2	227 bp	1.0	43.3
ycf1_p2	TGATGTTGATTTTACTGCAACCT	CTATCGCGGCCAAATCAAAA	34.8	45.0	57.0	57.2	214 bp	1.0	43.2
ycf1_p3	TGATGTTGATTTTACTGCAACCT	CCTATCGCGGCCAAATCAAA	34.8	50.0	57.0	59.0	215 bp	1.0	42.2
ycf1_p4	TGATGTTGATTTTACTGCAACCT	CTATCGCGGCCAAATCAAAAA	34.8	42.9	57.0	57.9	214 bp	1.0	42.2
ycf1_p5	TGATGTTGATTTTACTGCAACCT	TTATTGAACGGACCCTATCGC	34.8	47.6	57.0	57.9	228 bp	1.0	42.1