Hotspot

rpoC1

LSC · 22729-25637 · 2909 bp

Back to Castanopsis

Pi 0.00212

MarkerSeek score 58.20

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2909
variable_sites: 23
pi: 0.00212
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	ACCAGAGAGACACTAATTCACA	CCAGATGAATAGGAAGGAAGCT	40.9	45.5	56.5	57.3	2998-3000 bp	0.272727	11.4
rpoC1_p2	ACCAGAGAGACACTAATTCACA	TCCAGATGAATAGGAAGGAAGC	40.9	45.5	56.5	57.3	2999-3000 bp	0.181818	7.6
rpoC1_p3	AACCAGAGAGACACTAATTCACA	CCAGATGAATAGGAAGGAAGCT	39.1	45.5	57.2	57.3	2999-3000 bp	0.181818	7.6
rpoC1_p4	AACCAGAGAGACACTAATTCACA	TCCAGATGAATAGGAAGGAAGC	39.1	45.5	57.2	57.3	3000 bp	0.090909	3.6
rpoC1_p5	ACCAGAGAGACACTAATTCACA	TTCCAGATGAATAGGAAGGAAGC	40.9	43.5	56.5	58.0	3000 bp	0.090909	3.6