Hotspot

ndhI

SSC · 125570-126172 · 603 bp

Back to Castanopsis

Pi 0.00175

MarkerSeek score 59.50

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 603
variable_sites: 3
pi: 0.00175
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AGAAACCGCATTCCTTTCGA	TTTCCAACTCAGTTCGCTGT	45.0	45.0	57.8	57.7	703-715 bp	1.0	80.0
ndhI_p2	CGCATTCCTTTCGAGCAAAA	TTTCCAACTCAGTTCGCTGT	45.0	45.0	57.7	57.7	697-709 bp	1.0	78.6
ndhI_p3	GAAACCGCATTCCTTTCGAG	TTTCCAACTCAGTTCGCTGT	50.0	45.0	57.5	57.7	702-714 bp	1.0	77.1
ndhI_p4	ACGAAAGAAACCGCATTCCT	TTTCCAACTCAGTTCGCTGT	45.0	45.0	58.1	57.7	708-720 bp	0.909091	76.6
ndhI_p5	ACGAAAGAAACCGCATTCCT	TCTTTCCAACTCAGTTCGCT	45.0	45.0	58.1	57.1	710-722 bp	0.909091	71.9