Hotspot

ndhG

SSC · 124654-125199 · 546 bp

Back to Castanopsis

Pi 0.00253

MarkerSeek score 30.70

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 546
variable_sites: 4
pi: 0.00253
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	CATGCTAGAGGGCCAAGATG	ATCAAATTGCCTTGGGTCGT	55.0	45.0	58.1	58.1	1707-1749 bp	1.0	82.9
ndhG_p2	CATGCTAGAGGGCCAAGATG	AAAATGTTCCTGGTGGGGTC	55.0	50.0	58.1	58.0	1286-1317 bp	1.0	82.2
ndhG_p3	CCGCTTCTGGATCGATTCAT	AAAATGTTCCTGGTGGGGTC	50.0	50.0	57.8	58.0	1303-1334 bp	1.0	81.8
ndhG_p4	TCATGCTAGAGGGCCAAGAT	AAAATGTTCCTGGTGGGGTC	50.0	50.0	58.2	58.0	1287-1318 bp	1.0	81.5
ndhG_p5	CATGCTAGAGGGCCAAGATG	AGGAATGCGGTTTCTTTCGT	55.0	45.0	58.1	58.1	1535-1566 bp	0.909091	77.3