Hotspot

ndhF

SSC · 115926-118193 · 2268 bp

Back to Castanopsis

Pi 0.00333

MarkerSeek score 39.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2268
variable_sites: 26
pi: 0.00333
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CAGAGTACCGTCACCCATTG	AACACAAGGCTACAATCGCT	55.0	45.0	58.0	57.8	2864-2887 bp	1.0	80.0
ndhF_p2	CAGAGTACCGTCACCCATTG	ACACAAGGCTACAATCGCTT	55.0	45.0	58.0	57.8	2863-2886 bp	1.0	80.0
ndhF_p3	CAGAGTACCGTCACCCATTG	AAGAACTGGCGAATCCGAAA	55.0	45.0	58.0	57.8	2706-2727 bp	1.0	79.9
ndhF_p4	ACGAAGGGGACTAAACAGGA	ACACAAGGCTACAATCGCTT	50.0	45.0	58.0	57.8	2763-2777 bp	1.0	79.7
ndhF_p5	ACGAAGGGGACTAAACAGGA	AAGAACTGGCGAATCCGAAA	50.0	45.0	58.0	57.8	2606-2618 bp	1.0	79.6