Hotspot

ndhI-ndhA

SSC · 126173-127749 · 1577 bp

Back to Castanopsis

Pi 0.00339

MarkerSeek score 63.10

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 1577
variable_sites: 19
pi: 0.00339
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TCTTGTTTGAGGCAAGCCAT	CTCTACGTGCGATTCGTTGA	45.0	50.0	58.0	58.1	1633-1639 bp	1.0	81.0
ndhI-ndhA_p2	TCTTGTTTGAGGCAAGCCAT	TCTCTACGTGCGATTCGTTG	45.0	50.0	58.0	58.1	1634-1640 bp	1.0	81.0
ndhI-ndhA_p3	TCTTGTTTGAGGCAAGCCAT	CGGCTGCTCAATCGATTAGT	45.0	50.0	58.0	58.1	1690-1696 bp	1.0	80.7
ndhI-ndhA_p4	TCTCTTGTTTGAGGCAAGCC	CTCTACGTGCGATTCGTTGA	50.0	50.0	58.4	58.1	1635-1641 bp	1.0	77.9
ndhI-ndhA_p5	TCTCTTGTTTGAGGCAAGCC	TCTCTACGTGCGATTCGTTG	50.0	50.0	58.4	58.1	1636-1642 bp	1.0	77.9