Hotspot

ccsA-ndhD

SSC · 121620-121919 · 300 bp

Back to Castanopsis

Pi 0.00776

MarkerSeek score 35.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 300
variable_sites: 8
pi: 0.00776
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTCTGCAATTGTAGCCTCCC	TCAGTTGACAAGGTCGAAACT	50.0	42.9	57.9	57.5	478-482 bp	1.0	68.2
ccsA-ndhD_p2	TTCTGCAATTGTAGCCTCCC	AGTTGACAAGGTCGAAACTCT	50.0	42.9	57.9	57.2	476-480 bp	1.0	65.9
ccsA-ndhD_p3	TTCTGCAATTGTAGCCTCCC	CAGTTGACAAGGTCGAAACTC	50.0	47.6	57.9	57.1	477-481 bp	1.0	65.6
ccsA-ndhD_p4	CTGCAATTGTAGCCTCCCTG	TCAGTTGACAAGGTCGAAACT	55.0	42.9	58.6	57.5	476-480 bp	1.0	64.3
ccsA-ndhD_p5	TTCTGCAATTGTAGCCTCCC	TCAGTTGACAAGGTCGAAAC	50.0	45.0	57.9	55.9	478-482 bp	1.0	64.0