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psbE-petL

LSC · 70148-71394 · 1247 bp

Back to Castanopsis

Pi 0.00374

MarkerSeek score 61.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1247
variable_sites: 19
pi: 0.00374
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGTTCAACCCG	50.0	47.6	57.8	58.0	1449-1466 bp	1.0	74.0
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGTTCAACCCGA	50.0	47.6	57.8	58.0	1448-1465 bp	1.0	73.9
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	AGTCGTATCTTGTTCAACCCG	55.0	47.6	58.2	58.0	1444-1461 bp	1.0	73.6
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGTTCAACCCGA	55.0	47.6	58.2	58.0	1443-1460 bp	1.0	73.6
psbE-petL_p5	CTCCTGTGCTTCCAGACATG	AGTCGTATCTTGTTCAACCCG	55.0	47.6	58.3	58.0	1441-1458 bp	1.0	73.1